After incubation, every plate was observed carefully. All the plates were then incubated at 37☌ for 20 to 36 hours. 0.2 microliter and 10:1 dilutions of the samples were spread on Nutrient agar plate and different selective media. Nutrient agar media (Peptone 5 g/L, NaCl 5 g/L, Beef extract 3 g/L, Agar 15 g/L) are used for total heterotrophic count of organisms. Three different selective agar media MacConkey, Xylose-Lysine-Deoxycholate or XLD and M-FC agar media were used for isolation of Escherichia coli, Enterobacter, Staphylococcus aureus, Pseudomonas and Vibrio species, the most abundant and severe pathogens in drinking water, respectively. The study endeavored to answer four objectives:Ģ.2.

alginolyticus) from mineral water in Dhaka city. Escherichia coli, Enterobacter, Staphylococcus aureus, Pseudomonas and Vibrio species ( V. The present study was conducted to identify fecal coliforms and pathogenic bacteria e.g. Health effects connected with water supplies in developing nations are assessed to be based on four bacterial markers of tropical drinking-water quality (fecal coliforms, Escherichia coli, Enterococci and fecal Streptococci) and their relationship to the prevalence of diarrheal disease in Cebu, Philippines. According to Bangladesh health and injury report on children under 5 in 2005, children die every year from diarrhea. Water borne diseases constitute a major health burden in Bangladesh. The pathogenic most frequently transmitted though water, are those which cause infection of the intestinal tract, namely typhoid, paratyphoid diarrhea, dysentery and cholera. Among waterborne infections of bacterial origin typhoid fever, bacillary dysentery and diarrhea are normal in Bangladesh. In the disease-prone, humid, tropical region of Bangladesh, episodes of diarrheal diseases, frequently on a pandemic scale, are not uncommon and the conceivable part of water-borne pathogens in these outbreaks has been underlined. According to the World Health Report (2002), every year more than 3.4 million individuals die as a result of water related maladies demonstrating these as the leading cause of disease and death around the world. The increase also has happened in the countries where tap water is used as drinking water. The consumption of mineral water has been substantially increasing all over the world in since decade.

Gelatin hydrolysis test reagent